Estrategias de comunicación para el programa “Justicia terapéutica con enfoque restaurativo” en tiempos de COVID-19

La pandemia del COVID-19 y la cuarentena han propiciado grandes transformaciones en la manera cómo funcionan las sociedades. En este escenario, el reto reside en la capacidad de adaptación que tienen las personas y organizaciones, para afrontar los desafíos de la coyuntura actual. Como claro ejemplo del esfuerzo realizado para dar continuidad a una iniciativa creada para promover el bienestar en la sociedad, se presenta en este documento, el caso de la implementación del programa “Justicia Terapéutica con Enfoque Restaurativo”. En el cual se proponen estrategias de comunicación para poder potenciar el proceso de intervención en los beneficiarios del programa

Precision of test methods to assess the release of organic substances from construction products

The precision of the methods developed by the European Committee for Standardization (CEN) to assess the release of dangerous organic substances from construction products was evaluated as part of the validation of the methods aiming to convert them in EU standards. This evaluation of precision was done by an interlaboratory comparison organised by the Joint Research Centre (JRC) of the European Commission, in support of the Regulation 305/2011/EU (Construction Products Regulation). The present study focused on organic substances since inorganic substances were studied in previous work. The objective of this work was to evaluate for organic substances the precision (repeatability and reproducibility) of the methods developed by CEN Technical Committee 351 on dynamic surface leaching and up-flow percolation procedures from construction products, analysis of leachates/eluates and content analysis. This was done by comparing the results obtained by different laboratories when they analysed samples obtained from the same materials using the CEN methods under validation. The materials and analytes were proposed by CEN Technical Committee 351 to cover a reasonable range of products and substances at measurable levels. Validation plans were drawn up by the JRC in agreement with CEN/TC351. The tested substances were biocides, phthalates, mineral oil, polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs); and the selected construction products were render, sealant, asphalt aggregate and recycled aggregate. The methods assessed were horizontal dynamic surface leaching test CEN/TS 16637-2; horizontal up-flow percolation test CEN/TS 16637-3; content of organic substances – methods for extraction and analysis CEN/TS 17331; and analysis of organic substances in eluates CEN/TS 17332. Specific methods were used for the determination of biocides (WI 351035) and PAHs (WI 351034). Raw materials were obtained from industrial providers and processed to obtain laboratory samples at the JRC premises in Geel, Belgium. This interlaboratory test was open to expert laboratories from all Member States. Despite all efforts to recruit a higher number of participants, eventually 12 laboratories registered to the study and reported results; between 5 and 7 laboratories analysed each of the product following the CEN methods mentioned above. Precision of the reported results was evaluated according to ISO 5725-2. The obtained values for repeatability and reproducibility are shown in the annexes of this JRC report, together with the content levels, for each construction product and analyte within this study. For the dynamic surface leaching test CEN/TS 16637-2, relative repeatability standard deviation (RSDr) was 6 % and relative reproducibility standard deviations (RSDR) was 54 % (median values). Because of limited stability of biocides in water, it is recommended to assess their release at leaching times shorter than 64 days and to store the leachates in dark glass bottles at 4ºC. For the up-flow percolation test CEN/TS 16637-3, RSDr of 20 % and RSDR of 70 % were obtained as median values. For the analysis of eluates, RSDr were 2-32 % and RSDR were 23-51 %. And for content analysis, the values for RSDr are 6-9 % and for RSDR 27-63%. Due to the limited number of participants, any conclusion or recommendation must be made with the utmost care. However, despite the limited number of participants which might affect the robustness of this study, the results obtained for organic substances are consistent with the ones obtained for inorganic substances. Therefore it seems reasonable to incorporate the RSD values for organic substances to the standard methods.JRC.F.6-Reference Material

Bottom Effect in Atomic Force Microscopy Nanomechanics

In this work, the influence of the rigid substrate on the determination of the sample Young''s modulus, the so-called bottom-effect artifact, is demonstrated by an atomic force microscopy force-spectroscopy experiment. The nanomechanical properties of a one-component supported lipid membrane (SLM) exhibiting areas of two different thicknesses are studied: While a standard contact mechanics model (Sneddon) provides two different elastic moduli for these two morphologies, it is shown that Garcia''s bottom-effect artifact correction yields a unique value, as expected for an intrinsic material property. Remarkably, it is demonstrated that the ratio between the contact radius (and not only the indentation) and the sample thickness is the key parameter addressing the relevance of the bottom-effect artifact. The experimental results are validated by finite element method simulations providing a solid support to Garcia''s theory. The amphiphilic nature of the investigated material is representative of several kinds of lipids, suggesting that the results have far reaching implications for determining the correct Young''s modulus of SLMs. The generality of Garcia''s bottom-effect artifact correction allows its application to every kind of supported soft film

Influence of the disposition of the anisotropy axes into the magnetic properties of Mn-III dinuclear compounds with benzoato derivative bridges

The two new Mn-III dinudear compounds [{Mn-(H2O)(phen)}(2)(mu-4-CH3C6H4COO)(2)(mu-O)](ClO4)(2)center dot 3CH(3)CN center dot H2O (1.3CH(3)CN center dot H2O) and [{Mn(H2O)(phen)}(mu-O)](mu-2-BrC6H4COO)(2){Mn(NO3)(phen)}]NO3 (2) have been synthesized. Their structural data reveal significant differences in the shape of the coordination octahedron around the Mn-III ions in both compounds. The different distortions from ideal geometry incite a very different magnetic behavior, affecting both the zero-field splitting parameters of the Mn-III ions (D-Mn and E-Mn) and the magnetic interaction between them. Compound 1, with elongation in the monodentate ligand direction, shows antiferromagnetic coupling (ground state S = 0) and local D-Mn 0. Theoretical CASSCF and DFT calculations corroborate the different magnetic anisotropy and exchange coupling found in both compounds. Moreover, with the help of theoretical calculations, some interesting magneto-structural correlations have been found between the degree of distortion of the coordination octahedra and the magnetic coupling; it becomes more antiferromagnetic when the elongation parameter, Delta, in absolute value is increased

New role of the antidepressant imipramine as a Fascin1 inhibitor in colorectal cancer cells

Colorectal cancer: Antitumor antidepressant The antidepressant drug imipramine can block the activity of a protein that contributes to the progression of certain aggressive tumors. Serrated adenocarcinoma (SAC) is a form of colorectal cancer with a poor prognosis. A key factor in SAC development is the overexpression of the protein fascin1, which promotes the formation of structures that help cancer cells move around, thereby leading to metastasis. Pablo Conesa-Zamora at Santa Lucia University Hospital in Cartagena, Horacio Perez-Sanchez at the Universidad Catolica de Murcia in Guadalupe, Spain, and coworkers demonstrated that imipramine shows promise in binding to fascin1 and blocking its activity. The team analyzed over 9500 compounds as potential fascin1 blockers, identifying imipramine as a possible option. In tests on human tissues and in vivo studies using zebrafish, the drug reduced cancer invasion and metastasis. Serrated adenocarcinoma (SAC) is more invasive, has worse outcomes than conventional colorectal carcinoma (CRC), and is characterized by frequent resistance to anti-epidermal growth factor receptor (EGFR) and overexpression of fascin1, a key protein in actin bundling that plays a causative role in tumor invasion and is overexpressed in different cancer types with poor prognosis. In silico screening of 9591 compounds, including 2037 approved by the Food and Drug Administration (FDA), was performed, and selected compounds were analyzed for their fascin1 binding affinity by differential scanning fluorescence. The results were compared with migrastatin as a typical fascin1 inhibitor. In silico screening and differential scanning fluorescence yielded the FDA-approved antidepressant imipramine as the most evident potential fascin1 blocker. Biophysical and different in vitro actin-bundling assays confirm this activity. Subsequent assays investigating lamellipodia formation and migration and invasion of colorectal cancer cells in vitro using 3D human tissue demonstrated anti-fascin1 and anti-invasive activities of imipramine. Furthermore, expression profiling suggests the activity of imipramine on the actin cytoskeleton. Moreover, in vivo studies using a zebrafish invasion model showed that imipramine is tolerated, its anti-invasive and antimetastatic activities are dose-dependent, and it is associated with both constitutive and induced fascin1 expression. This is the first study that demonstrates an antitumoral role of imipramine as a fascin1 inhibitor and constitutes a foundation for a molecular targeted therapy for SAC and other fascin1-overexpressing tumors.Peer reviewe

Factorial Equivalence of the Social Skills Scale in the Lambayeque University Context According to Sex

This study aimed to show whether the theoretical model of the social skills construct, proposed by Gismero (2000), is invariant in male and female samples of Peruvian university students from Lambayeque. In order to meet the proposed objective, a non-experimental, cross-sectional, comparative psychometric design by hierarchical confirmatory procedures was chosen. Two samples of university students were used, one of 1,084 female students and the other of 1,043 male students. Both groups were applied the self-report of social skills (EHS). With the data obtained, a confirmatory factor analysis of the first-order factor structure of the social skills construct was performed in each group, based on the polychoric correlation matrix. The invariance of the scale according to sex was evaluated on the basis of the second-order factor structure, by means of multigroup confirmatory factor analysis of nested models. No significant differences in the fit to the data were found between the two-factor second-order factor structure and the alternative one-factor model. Consecutive tests of configurational invariance, disturbance, and first- and second-order metrics gave evidence, respectively, that the model structure, second-order factor errors, and first- and second-order loadings are equivalent in the two study samples. With the data from the study, it is concluded that the structure of the EHS scale is invariant to the sex variable in the Lambayeque context

New 1-aryl-3-substituted propanol derivatives as antimalarial agents

This paper describes the synthesis and in vitro antimalarial activity against a P. falciparum 3D7 strain of some new 1-aryl-3-substituted propanol derivatives. Twelve of the tested compounds showed an IC50 lower than 1 μM. These compounds were also tested for cytotoxicity in murine J774 macrophages. The most active compounds were evaluated for in vivo activity against P. berghei in a 4-day suppressive test. Compound 12 inhibited more than 50% of parasite growth at a dose of 50 mg/kg/day. In addition, an FBIT test was performed to measure the ability to inhibit ferriprotoporphyrin biocrystallization. This data indicates that 1-aryl-3-substituted propanol derivatives hold promise as a new therapeutic option for the treatment of malaria

Expression atlas of the Deubiquitinating enzymes in the adult mouse retina, their evolutionary diversification and phenotypic roles

Ubiquitination is a relevant cell regulatory mechanism to determine protein fate and function. Most data has focused on the role of ubiquitin as a tag molecule to target substrates to proteasome degradation, and on its impact in the control of cell cycle, protein homeostasis and cancer. Only recently, systematic assays have pointed to the relevance of the ubiquitin pathway in the development and differentiation of tissues and organs, and its implication in hereditary diseases. Moreover, although the activity and composition of ubiquitin ligases has been largely addressed, the role of the deubiquitinating enzymes (DUBs) in specific tissues, such as the retina, remains mainly unknown. In this work, we undertook a systematic analysis of the transcriptional levels of DUB genes in the adult mouse retina by RT-qPCR and analyzed the expression pattern by in situ hybridization and fluorescent immunohistochemistry, thus providing a unique spatial reference map of retinal DUB expression. We also performed a systematic phylogenetic analysis to understand the origin and the presence/absence of DUB genes in the genomes of diverse animal taxa that represent most of the known animal diversity. The expression landscape obtained supports the potential subfunctionalization of paralogs in those families that expanded in vertebrates. Overall, our results constitute a reference framework for further characterization of the DUB roles in the retina and suggest new candidates for inherited retinal disorders